Journal of Biomechanics
Volume 38, Issue 5 , Pages 1093-1105 , May 2005

High rate shear strain of three-dimensional neural cell cultures: a new in vitro traumatic brain injury model

  • Michelle C. LaPlaca

      Affiliations

    • Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive, Atlanta GA, USA
    • Corresponding Author InformationCorresponding author. Laboratory for Neuroengineering, Georgia Institute of Technology, 313 Ferst Drive, Atlanta, GA 30332-0535, USA. Tel.: +1-404-385-0629; fax: +1-404-385-5044
    • ,
  • D.Kacy Cullen

      Affiliations

    • Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive, Atlanta GA, USA
    • ,
  • Justin J. McLoughlin

      Affiliations

    • Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 801 Ferst Drive, Atlanta GA, USA
    • ,
  • Robert S. Cargill II

      Affiliations

    • Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 801 Ferst Drive, Atlanta GA, USA
    • Present address: Exponent Failure Analysis Associates, 3401 Market Street, Suite 300, Philadelphia, PA 19104, USA

,Accepted 20 May 2004.

References 

  1. Balentine JD, Greene WB, Bornstein M. In vitro spinal cord trauma. Laboratory Investigation. 1988;58(1):93–99
  2. Cargill RS, Thibault LE. Acute alterations in [Ca2+]i in NG108-15 cells subjected to high strain rate deformation and chemical hypoxia (an in vitro model for neural trauma). Journal of Neurotrauma. 1996;13(7):395–407
  3. Chu CS, Lin MS, Huang HM, Lee MC. Finite element analysis of cerebral contusion. Journal of Biomechanics. 1994;27(2):187–194
  4. Cukierman E, Pankov R, Stevens DR, Yamada KM. Taking cell-matrix adhesions to the third dimension. Science. 2001;294(5547):1708–1712
  5. Cukierman E, Pankov R, Yamada KM. Cell interactions with three-dimensional matrices. Current Opinion in Cell Biology. 2002;14(5):633–639
  6. Donnelly BR, Medige J. Shear properties of human brain tissue. Journal of Biomechanical Engineering. 1997;119(4):423–432
  7. Ellis EF, McKinney JS, Willoughby KA, Liang S, Povlishock JT. A new model for rapid stretch-induced injury of cells in culture (characterization of the model using astrocytes). Journal of Neurotrauma. 1995;12(3):325–339
  8. Fawcett JW, Housden E, Smith-Thomas L, Meyer RL. The growth of axons in three-dimensional astrocyte cultures. Developmental Biology. 1989;135(2):449–458
  9. Fawcett JW, Barker RA, Dunnett SB. Dopaminergic neuronal survival and the effects of bFGF in explant, three dimensional and monolayer cultures of embryonic rat ventral mesencephalon. Experimental Brain Research. 1995;106:275–282
  10. Flanagan LA, Ju YE, Marg B, Osterfield M, Janmey PA. Neurite branching on deformable substrates. Neuroreport. 2002;13(18):2411–2415
  11. Geddes DM, Cargill RS. An in vitro model of neural trauma (device characterization and calcium response to mechanical stretch). Journal of Biomechanical Engineering. 2001;123(3):247–255
  12. Gennarelli TA. Mechanisms of brain injury. Journal of Emergency Medicine. 1993;11(Suppl 1):5–11
  13. Gennarelli TA. Animate models of human head injury. Journal of Neurotrauma. 1994;11:357–368
  14. Gennarelli TA, Thibault LE, Adams JH, Graham DI, Thompson CJ, Marcincin RP. Diffuse axonal injury and traumatic coma in the primate. Annals of Neurology. 1982;12(6):564–574
  15. Granet C, Laroche N, Vico L, Alexandre C, Lafage-Proust MH. Rotating-wall vessels, promising bioreactors for osteoblastic cell culture (comparison with other 3D conditions). Medical and Biological Engineering and Computing. 1998;36(4):513–519
  16. Gross GW, Lucas JH, Higgins ML. Laser microbeam surgery (ultrastructural changes associated with neurite transection in culture). The Journal of Neuroscience. 1983;3(10):1979–1993
  17. Gumbiner BM, Yamada KM. Cell-to-cell contact and extracellular matrix. Current Opinion in Cell Biology. 1995;7(5):615–618
  18. Holburn AH. Mechanics of head injuries. Lancet. 1943;2:438–441
  19. LaPlaca MC, Thibault LE. An in vitro traumatic injury model to examine the response of neurons to a hydrodynamically induced deformation. Annals of Biomedical Engineering. 1997;25(4):665–677
  20. Lucas JH, Gross GW, Emery DG, Gardner CR. Neuronal survival or death after dendrite transection close to the perikaryon (correlation with electrophysiologic, morphologic, and ultrastructural changes). Central Nervous Systems Trauma. 1985;2(4):231–255
  21. Margulies SS, Thibault LE. A proposed tolerance criteria for diffuse axonal injury in man. Journal of Biomechanics. 1992;25(8):917–923
  22. Margulies SS, Thibault LE, Gennarelli TA. Physical model simulations of brain injury in the primate. Journal of Biomechanics. 1990;23(8):823–836
  23. Morrison BR, Meaney DF, McIntosh TK. Mechanical characterization of an in vitro device designed to quantitatively injure living brain tissue. Annals of Biomedical Engineering. 1998;26(3):381–390
  24. Morrison BR, Saatman KE, Meaney DF, McIntosh TK. In vitro central nervous system models of mechanically induced trauma (a review). Journal of Neurotrauma. 1998;15(11):911–928
  25. Mukhin A, Fan L, Faden AI. Activation of metabotropic glutamate receptor subtype mGluR1 contributes to post-traumatic neuronal injury. Journal of Neuroscience. 1996;16(19):6012–6020
  26. Murphy EJ, Horrocks LA. A model for compression trauma (pressure-induced injury in cell cultures). Journal of Neurotrauma. 1993;10(4):431–444
  27. Ogden, R.W., 1984. Non-linear Elastic Deformations. Halsted Press, New York, 532pp.
  28. Ommaya AK, Thibault L, Bandak FA. Mechanisms of impact head injury. International Journal of Impact Engineering. 1994;15(4):535–560
  29. Regan RF, Choi DW. The effect of NMDA, AMPA/kainate, and calcium channel antagonists on traumatic cortical neuronal injury in culture. Brain Research. 1994;633:236–242
  30. Roberts I, Schierhout G, Alderson P. Absence of evidence for the effectiveness of five interventions routinely used in the intensive care management of severe head injury (a systematic review [see comments]). Journal of Neurology, Neurosurgery and Psychiatry. 1998;65(5):729–733
  31. Schmeichel KL, Bissell MJ. Modeling tissue-specific signaling and organ function in three dimensions. Journal of Cell Science. 2003;116(Part 12):2377–2388
  32. Schwab ME, Bartholdi D. Degeneration and regeneration of axons in the lesioned spinal cord. Physiological Review. 1996;76(2):319–370
  33. Shepard SR, Ghajar JBG, Giannuzzi R, Kupferman S, Hariri RJ. Fluid percussion barotrauma chamber (a new in vitro model for traumatic brain injury). Journal of Surgical Research. 1991;51:417–424
  34. Shuck, L.Z., Advani, S.H., 1972. Rheological response of human brain tissue in shear. Journal of Basic Engineering 905–911.
  35. Sieg F, Wahle P, Pape H-C. Cellular reactivity to mechanical axonal injury in an organotypic in vitro model of neurotrauma. Journal of Neurotrauma. 1999;16(12):1197–1213
  36. Sievers C, Platt N, Perry VH, Coleman MP, Conforti L. Neurites undergoing Wallerian degeneration show an apoptotic-like process with Annexin V positive staining and loss of mitochondrial membrane potential. Neuroscience Research. 2003;46(2):161–169
  37. Takeshita K, Bowen WC, Michalopoulos GK. Three-dimensional culture of hepatocytes in a continuously flowing medium. In Vitro Cellular and Development Biology-Animal. 1998;34(6):482–485
  38. Tecoma ES, Monyer H, Goldberg MP, Choi DW. Traumatic neuronal injury is attenuated by NMDA antagonists. Neuron. 1989;2(6):1541–1545
  39. Wallis RA, Panizzon KL. Felbamate neuroprotection against CA1 traumatic neuronal injury. European Journal of Pharmacology. 1995;294(2–3):475–482
  40. Wang F, Weaver VM, Petersen OW, Larabell CA, Dedhar S, Briand P, et al. Reciprocal interactions between beta1-integrin and epidermal growth factor receptor in three-dimensional basement membrane breast cultures (a different perspective in epithelial biology). Proceedings of the National Academy of Science USA. 1998;95(25):14821–14826
  41. Yamada KM, Pankov R, Cukierman E. Dimensions and dynamics in integrin function. Brazilian Journal of Medical and Biological Research. 2003;36(8):959–966

PII: S0021-9290(04)00265-9

doi: 10.1016/j.jbiomech.2004.05.032

Journal of Biomechanics
Volume 38, Issue 5 , Pages 1093-1105 , May 2005

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