Journal of Biomechanics
Volume 39, Issue 8 , Pages 1419-1427 , 2006

Mechanical stimulation effects on functional end effectors in osteoblastic MG-63 cells

  • M.M. Saunders

      Affiliations

    • Division of Musculoskeletal Sciences and Center for Biomedical Devices and Functional Tissue Engineering, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, PA 17033, USA
    • Corresponding Author InformationCorresponding author. Tel.: +17175314818; fax: +17175317583.
    • ,
  • A.F. Taylor

      Affiliations

    • Division of Musculoskeletal Sciences and Center for Biomedical Devices and Functional Tissue Engineering, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, PA 17033, USA
    • ,
  • C. Du

      Affiliations

    • Division of Musculoskeletal Sciences and Center for Biomedical Devices and Functional Tissue Engineering, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, PA 17033, USA
    • ,
  • Z. Zhou

      Affiliations

    • Division of Musculoskeletal Sciences and Center for Biomedical Devices and Functional Tissue Engineering, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, PA 17033, USA
    • ,
  • V.D. Pellegrini Jr.

      Affiliations

    • Department of Orthopaedics, University of Maryland School of Medicine, 22 South Greene Street, Suite S 11B, Baltimore, MD 21201, USA
    • ,
  • H.J. Donahue

      Affiliations

    • Division of Musculoskeletal Sciences and Center for Biomedical Devices and Functional Tissue Engineering, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, PA 17033, USA

,Accepted 8 April 2005.

References 

  1. Aubin JE, Bonnelye E. Osteoprotegerin and its ligand: a new paradigm for regulation of osteoclastogenesis and bone resorption. Osteoporosis International. 2000;11:905–913
  2. Bottlang M, Simnacher M, Schmidt H, Brand RA, Claes L. A cell strain system for small homogenous strain applications. Biomedizinische Technik. 1997;42:305–309
  3. Brown TD, Bottlang M, Pedersen DR, Banes AJ. Loading paradigms—intentional and unintentional—for cell culture mechanostimulus. American Journal of the Medical Sciences. 1998;316:162–168
  4. Cecchini MG, Dominguez MG, Mocci S, Wetterwald A, Felix R, Fleisch H, et al. Role of colony stimulating factor-1 in the establishment and regulation of tissue macrophages during postnatal development of the mouse. Development. 1994;120(6):1357–1372
  5. Everts V, Delaisse JM, Korper W, Jansen DC, Tigchelaar-Gutter W, Saftig P, et al. The bone lining cell: its role in cleaning Howship's Lacunae and initiating bone formation. Journal of Bone and Mineral Research. 2002;17(1):77–90
  6. Hofbauer LC. Osteoprotegerin ligand and osteoprotegerin: novel implications for osteoclast biology and bone metabolism. European Journal of Endocrinology. 1999;141:195–210
  7. Hung CT, Pollack SR, Reilly TM, Brighton CT. Real-time calcium response of cultured bone cells to fluid flow. Clinical Orthopaedics and Related Research. 1995;313:256–269
  8. Hung CT, Allen FD, Pollack SR, Brighton CT. Intracellular Ca2+ Stores and extracellular Ca2+ are required in the real-time Ca2+ response of bone cells experiencing fluid flow. Journal of Biomechanics. 1996;29:1411–1417
  9. Imamura K, Ozawa H, Hiraide T, Takahashi N, Shibasaki Y, Fukuhara T, et al. Continuously applied compressive pressure induces bone resorption by a mechanism involving prostaglandin E2 synthesis. Journal of Cellular Physiology. 1990;144:222–228
  10. Jacobs CR, Yellowley CE, Davis BR, Zhou Z, Donahue HJ. Differential effect of steady versus oscillating flow on bone cells. Journal of Biomechanics. 1998;31:969–976
  11. Jee WS, Ueno K, Deng YP, Woodbury DM. The effects of prostaglandin E2 in growing rats: increased metaphyseal hard tissue and cortico-endosteal bone formation. Calcified Tissue International. 1985;37:148–157
  12. Kanzaki H, Chiba M, Shimizu Y, Mitani H. Periodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor kappaB ligand up-regulation via prostaglandin E2 synthesis. Journal of Bone and Mineral Research. 2002;17(2):210–220
  13. Klein-Nulend J, Burger EH, Semeins CM, Raisz LG, Pilbeam CC. Pulsating fluid flow stimulates prostaglandin release and inducible prostaglandin G/H synthase mRNA expression in primary mouse bone cells. Journal of Bone and Mineral Research. 1997;12:45–51
  14. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680–685
  15. Li Z, Zhou Z, Daniel EE, O’Byrne PM. Expression of gap junction protein Cx43 and mRNA in canine trachea smooth muscle, bronchus and lung. American Review of Respiratory Diseases. 1993;147:4;A53
  16. Liegibel UM, Sommer U, Tomakidi P, Hilscher U, van den Heuvel L, Pirzer R, et al. Concerted action of androgens and mechanical strain shifts bone metabolism from high turnover into an osteoanabolic mode. Journal of Experimental Medicine. 2002;196(10):1387–1392
  17. Martin B, Burr DB, Sharkey N. Mechanical adaptability of the skeleton. In:  Martin B editors. Skeletal Tissue Mechanics. Berlin: Springer; 1998;p. 225–272
  18. Mosely JR, March BM, Lynch J, Lanyon LE. Strain magnitude related changes in whole bone architecture in growing rats. Bone. 1997;20:191–198
  19. Owan I, Burr DB, Turner CH, Qui J, Tu Y, Onyia JE, et al. Mechanotransduction in bone: osteoblasts are more responsive to fluid forces than mechanical strain. American Journal of Physiology—Cell Physiology. 1997;273(3 Pt 1):C810–C815
  20. Pitsillides AA, Rawlinson SC, Suswillo RF, Bourrin S, Zaman G, Lanyon LE. Mechanical strain-induced NO production by bone cells: a possible role in adaptive bone (re)modeling?. FASEB Journal. 1995;9:1614–1622
  21. Rubin CT, Lanyon LE. Regulation of bone formation by applied dynamic loads. Journal of Bone and Joint Surgery. 1984;66A:397–402
  22. Rubin J, Fan X, Biskobing DM, Taylor WR, Rubin CT. Osteoclastogenesis is repressed by mechanical strain in an in vitro model. Journal of Orthopaedic Research. 1999;17(5):639–645
  23. Rubin J, Murphy T, Nanes MS, Fan X. Mechanical strain inhibits expression of osteoclast differentiation by murine stromal cells. American Journal of Physiology—Cell Physiology. 2000;278(6):C1126–C1132
  24. Saunders MM, Donahue HJ. Development of a cost-effective loading machine for biomechanical evaluation of mouse transgenic models. Medical Engineering and Physics. 2004;26:595–603
  25. Saunders MM, You J, Trosko JE, Yamasaki H, Donahue HJ, Jacobs CR. Gap junctions and fluid flow response in MC3T3-E1 cells. American Journal of Physiology—Cell Physiology. 2001;281(6):1917–1925
  26. Saunders MM, You J, Zhou Z, Li Z, Yellowley CE, Kunze E, et al. Fluid-flow induced prostaglandin E2 response of osteoblastic ROS 17/2.8 cells is gap junction-mediated and independent of cytosolic calcium. Bone. 2003;32:350–356
  27. Shalhoub V, Faust J, Boyle WJ, Dunstan CR, Kelley M, Kaufman S, et al. OPG and OPGL effects on osteoclast formation from human peripheral blood mononuclear cell precursors. Journal of Cellular Biochemistry. 1999;72(2):251–261
  28. Stanley, E.R., 1994. In: Thomson, A. (Ed.), The Cytokine Handbook, second ed. Academic Press, New York, p. 387.
  29. Suda T, Udagawa N, Takahashi N. Cells of bone: osteoclast generation. In:  Bilezikian JP,  Raisz LG,  Rodan GA editor. Principles of Bone Biology. New York: Academic Press; 1996;p. 87–102
  30. Suda T, Takahashi N, Ugadawa N, Jimi E, Gillespie MT, Martin TJ. Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocrine Reviews. 1999;20:345–357
  31. You J, Yellowley CE, Donahue HJ, Zhang Y, Chen Q, Jacobs CR. Substrate deformation levels associated with routine physical activity are less stimulatory to bone cells relative to loading-induced oscillatory fluid flow. Journal of Biomechanical Engineering. 2000;122:387–393
  32. You J, Zhen X, Yellowley CE, Chen Q, Donahue HJ, Jacobs CR. Osteopontin gene regulation by oscillatory fluid flow via intracellular calcium mobilization and activation of mitogen-activated protein kinase in MC3T3-E1 osteoblasts. Journal of Biological Chemistry. 2001;276(16):13365–13371

PII: S0021-9290(05)00180-6

doi: 10.1016/j.jbiomech.2005.04.011

Journal of Biomechanics
Volume 39, Issue 8 , Pages 1419-1427 , 2006

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