Changes of opening angle in hypertensive and hypotensive arteries in 3-day organ culture

Abstract 

To study the effect of pressure changes on the opening angle of arteries in organ culture, tubular segments of porcine common carotid arteries were cultured with pulsatile flow perfusion under hypertensive (150±20mmHg), normotensive (100±20mmHg), or hypotensive (30±10mmHg) pressure while maintaining the arteris at a physiological wall shear stress of ∼15dyn/cm² for up to 3 days. Arteries were then cut into short ring segments by sections perpendicular to the axis and then cut open radially to observe the opening angle in aerated phosphate buffered saline solution (37°C). Norepinephrine (NE, 10μM), carbacol (CCh, 100μM), and sodium nitroprusside (SNP, 10μM) were added after the radial cut at 30, 20, and 30min intervals, the opening angles were measured, respectively. Results show that hypertensive arteries developed a significantly larger opening angle than normotensive and hypotensive arteries, associated with a significant increase in cell proliferation. In addition, with smooth muscle contraction activated by NE, the opening angle decreases significantly in hypertensive arteries but has little change in hypotensive and normotensive arteries, indicating an enhancement of smooth muscle contraction on the lumen side of the hypertensive arterial wall. In comparison, hypotensive pressure has little effect on arterial opening angle and cell proliferation.

Keywords: Vasomotor, Opening angle, Hypertension, Vascular remodeling, Smooth muscle, Cell proliferation, Ex vivo, Artery culture, Organ culture, Porcine