Journal of Biomechanics
Volume 41, Issue 9 , Pages 1885-1891 , 2008

Influence of perfusion and cyclic compression on proliferation and differentiation of bone marrow stromal cells in 3-dimensional culture

  • M. Jagodzinski

      Affiliations

    • Department of Trauma, Hannover Medical School (MHH), Carl-Neuberg-Street 1, D-30625 Hanover, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +495115322278; fax: +495115325877.
  • ,
  • A. Breitbart

      Affiliations

    • Department of Trauma, Hannover Medical School (MHH), Carl-Neuberg-Street 1, D-30625 Hanover, Germany
  • ,
  • M. Wehmeier

      Affiliations

    • Department of Clinical Chemistry, Hannover Medical School (MHH), Hanover, Germany
  • ,
  • E. Hesse

      Affiliations

    • Department of Trauma, Hannover Medical School (MHH), Carl-Neuberg-Street 1, D-30625 Hanover, Germany
    • Current address: Department of Medicine, Harvard Medical School and Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA, USA.
  • ,
  • C. Haasper

      Affiliations

    • Department of Trauma, Hannover Medical School (MHH), Carl-Neuberg-Street 1, D-30625 Hanover, Germany
  • ,
  • C. Krettek

      Affiliations

    • Department of Trauma, Hannover Medical School (MHH), Carl-Neuberg-Street 1, D-30625 Hanover, Germany
  • ,
  • J. Zeichen

      Affiliations

    • Department of Trauma, Hannover Medical School (MHH), Carl-Neuberg-Street 1, D-30625 Hanover, Germany
  • ,
  • S. Hankemeier

      Affiliations

    • Department of Trauma, Hannover Medical School (MHH), Carl-Neuberg-Street 1, D-30625 Hanover, Germany

,Accepted 1 April 2008.

References 

  1. Allen FD, Hung CT, Pollack SR, Brighton CT. Serum modulates the intracellular calcium response of primary cultured bone cells to shear flow. Journal of Biomechanics. 2000;33:1585–1591
  2. Altman GH, Horan RL, Martin I, Farhadi J, Stark PR, Volloch V, et al. Cell differentiation by mechanical stress. FASEB Journal. 2002;16:270–272
  3. Angele P, Yoo JU, Smith C, Mansour J, Jepsen KJ, Nerlich M, et al. Cyclic hydrostatic pressure enhances the chondrogenic phenotype of human mesenchymal progenitor cells differentiated in vitro. Journal of Orthopaedic Research. 2003;21:451–457
  4. Bancroft GN, Sikavitsas VI, van den DJ, Sheffield TL, Ambrose CG, Jansen JA, et al. Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner. Proceedings of the National Academy of Sciences USA. 2002;99:12600–12605
  5. Banfi A, Bianchi G, Notaro R, Luzzatto L, Cancedda R, Quarto R. Replicative aging and gene expression in long-term cultures of human bone marrow stromal cells. Tissue Engineering. 2002;8:901–910
  6. Bertram H, Mayer H, Schliephake H. Effect of donor characteristics, technique of harvesting and in vitro processing on culturing of human marrow stromal cells for tissue engineered growth of bone. Clinical Oral Implants Research. 2005;16:524–531
  7. Brown TD. Techniques for mechanical stimulation of cells in vitro: a review. Journal of Biomechanics. 2000;33:3–14
  8. Caplan AI, Bruder SP. Mesenchymal stem cells: building blocks for molecular medicine in the 21st century. Trends in Molecular Medicine. 2001;7:259–264
  9. Cheng B, Kato Y, Zhao S, Luo J, Sprague E, Bonewald LF, et al. PGE(2) is essential for gap junction-mediated intercellular communication between osteocyte-like MLO-Y4 cells in response to mechanical strain. Endocrinology. 2001;142:3464–3473
  10. Datta N, Pham QP, Sharma U, Sikavitsas VI, Jansen JA, Mikos AG. In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation. Proceedings of the National Academy of Sciences USA. 2006;103:2488–2493
  11. Frank O, Heim M, Jakob M, Barbero A, Schafer D, Bendik I, et al. Real-time quantitative RT-PCR analysis of human bone marrow stromal cells during osteogenic differentiation in vitro. Journal of Cellular Biochemistry. 2002;85:737–746
  12. Gomes ME, Sikavitsas VI, Behravesh E, Reis RL, Mikos AG. Effect of flow perfusion on the osteogenic differentiation of bone marrow stromal cells cultured on starch-based 3-dimensional scaffolds. Journal of Biomedical Materials Research A. 2003;67:87–95
  13. Hankemeier S, Keus M, Zeichen J, Jagodzinski M, Barkhausen T, Bosch U, et al. Modulation of proliferation and differentiation of human bone marrow stromal cells by fibroblast growth factor 2: potential implications for tissue engineering of tendons and ligaments. Tissue Engineering. 2005;11:41–49
  14. Holtorf HL, Sheffield TL, Ambrose CG, Jansen JA, Mikos AG. Flow perfusion culture of marrow stromal cells seeded on porous biphasic calcium phosphate ceramics. Annals of Biomedical Engineering. 2005;33:1238–1248
  15. Igarashi A, Segoshi K, Sakai Y, Pan H, Kanawa M, Higashi Y, et al. Selection of common markers for bone marrow stromal cells from various bones using real-time RT-PCR: effects of passage number and donor age. Tissue Engineering. 2007;13:2405–2417
  16. Jagodzinski M, Drescher M, Zeichen J, Hankemeier S, Bosch U, van Griensven M. Effects of cyclic longitudinal mechanical strain and dexamethasone on osteogenic differentiation of human bone marrow stromal cells. European Cells and Materials. 2004;16:35–41
  17. Jagodzinski M, Hankemeier S, van Griensven M, Bosch U, Krettek C, Zeichen J. Influence of cyclic mechanical strain and heat of human tendon fibroblasts on HSP-72. European Journal of Applied Physiology. 2005;96:249–256
  18. Jasmund I, Bader A. Bioreactor developments for tissue engineering applications by the example of the bioartificial liver. Advances in Biochemical Engineering and Biotechnology. 2002;74:99–109
  19. Kaspar D, Seidl W, Neidlinger-Wilke C, Ignatius A, Claes L. Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity. Journal of Biomechanics. 2000;33:45–51
  20. Kasten P, Vogel J, Luginbuhl R, Niemeyer P, Weiss S, Schneider S, et al. Influence of platelet-rich plasma on osteogenic differentiation of mesenchymal stem cells and ectopic bone formation in calcium phosphate ceramics. Cells Tissues Organs. 2006;183:68–79
  21. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta C(T)) method. Methods. 2001;25:402–408
  22. Mauney JR, Sjostorm S, Blumberg J, Horan R, O’Leary JP, Vunjak-Novakovic G, et al. Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds in vitro. Calcified Tissue International. 2004;74:458–468
  23. Meyer U, Meyer T, Wiesmann HP, Stratmann U, Kruse-Losler B, Maas H, et al. The effect of magnitude and frequency of interfragmentary strain on the tissue response to distraction osteogenesis. Journal of Oral and Maxillofaciale Surgery. 1999;57:1331–1339
  24. Neidlinger-Wilke C, Grood ES, Wang JHC, Brand RA, Claes L. Cell alignment is induced by cyclic changes in cell length: studies of cells grown in cyclically stretched substrates. Journal of Orthopaedic Research. 2001;19:286–293
  25. Oliva A, Passaro I, Di Pasquale R, Di Feo A, Criscuolo M, Zappia V, et al. Ex vivo expansion of bone marrow stromal cells by platelet-rich plasma: a promising strategy in maxillo-facial surgery. International Journal of Immunopathology and Pharmacology. 2005;18:47–53
  26. Pittenger MF, Mosca JD, McIntosh KR. Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma. Current Topics in Microbiology and Immunology. 2000;251:3–11
  27. Runtemund A, Haasper C, Glasmacher B, Jagodzinski M. Control of Mechanical Stimulation of 3-dimensional Constructs in a Bioreactor. International Journal of Artificial Organs. 2007;30:730
  28. Saito T, Kuang JQ, Lin CC, Chiu RC. Transcoronary implantation of bone marrow stromal cells ameliorates cardiac function after myocardial infarction. Journal of Thoracic and Cardiovascular Surgery. 2003;126:114–123
  29. Siddappa R, Licht R, van Blitterswijk C, de Boer J. Donor variation and loss of multipotency during in vitro expansion of human mesenchymal stem cells for bone tissue engineering. Journal of Orthopedic Research. 2007;25:1029–1041
  30. Sikavitsas VI, Bancroft GN, Lemoine JJ, Liebschner MA, Dauner M, Mikos AG. Flow perfusion enhances the calcified matrix deposition of marrow stromal cells in biodegradable nonwoven fiber mesh scaffolds. Annals of Biomedical Engineering. 2005;33:63–70
  31. Stanford CM, Stevens JW, Brand RA. Cellular deformation reversibly depresses RT-PCR detectable levels of bone-related mRNA. Journal of Biomechanics. 1995;28:1419–1427
  32. Tanaka SM. A new mechanical stimulator for cultured bone cells using piezoelectric actuator. Journal of Biomechanics. 1999;32:427–430
  33. Trivedi P, Hematti P. Derivation and immunological characterization of mesenchymal stromal cells from human embryonic stem cells. Experimental Hematology. 2008;36:350–359
  34. Wolf S, Augat P, Eckert-Hubner K, Laule A, Krischak GD, Claes LE. Effects of high-frequency, low-magnitude mechanical stimulus on bone healing. Clinical Orthopaedics. 2001;385:192–198
  35. Wolff J. The Law of Bone Remodeling. Berlin Heidelberg, New York: Springer; 1892;
  36. Woodbury D, Reynolds K, Black IB. Adult bone marrow stromal stem cells express germline, ectodermal, endodermal, and mesodermal genes prior to neurogenesis. Journal of Neuroscience Research. 2002;69:908–917
  37. Yamaguchi A, Komori T, Suda T. Regulation of osteoblast differentiation mediated by bone morphogenetic proteins, hedgehogs, and Cbfa1. Endocrine Revviews. 2000;21:393–411
  38. Zeichen J, van Griensven M, Bosch U. The proliferative response of isolated human tendon fibroblasts to cyclic biaxial mechanical strain. American Journal of Sports Medicine. 2000;28:888–892

PII: S0021-9290(08)00170-X

doi: 10.1016/j.jbiomech.2008.04.001

Journal of Biomechanics
Volume 41, Issue 9 , Pages 1885-1891 , 2008