Compressive mechanical properties of the intraluminal thrombus in abdominal aortic aneurysms and fibrin-based thrombus mimics

Ashton, John H.; Vande Geest, Jonathan P.; Simon, Bruce R.; Haskett, Darren G.

doi:10.1016/j.jbiomech.2008.10.024

« Previous Next »Journal of Biomechanics
Volume 42, Issue 3 , Pages 197-201, 9 February 2009

Compressive mechanical properties of the intraluminal thrombus in abdominal aortic aneurysms and fibrin-based thrombus mimics

John H. Ashton
- Biomedical Engineering Interdisciplinary Program, University of Arizona, Tucson, AZ
Jonathan P. Vande Geest
- Biomedical Engineering Interdisciplinary Program, University of Arizona, Tucson, AZ
- BIO5 Institute, University of Arizona, Tucson, AZ
- Soft Tissue Biomechanics Laboratory, Department of Aerospace and Mechanical Engineering, University of Arizona, 1130 N Mountain Avenue, PO Box 210119, Tucson, AZ 85721-0119, USA
- Corresponding author at: Soft Tissue Biomechanics Laboratory, Department of Aerospace and Mechanical Engineering, University of Arizona, 1130 N Mountain Avenue, PO Box 210119, Tucson, AZ85721-0119, USA. Tel.: +15206212514; fax: +15206218191.
Bruce R. Simon
- Biomedical Engineering Interdisciplinary Program, University of Arizona, Tucson, AZ
- Soft Tissue Biomechanics Laboratory, Department of Aerospace and Mechanical Engineering, University of Arizona, 1130 N Mountain Avenue, PO Box 210119, Tucson, AZ 85721-0119, USA
Darren G. Haskett
- Department of Agriculture and Bio Systems Engineering, University of Arizona, Tucson, AZ

Accepted 19 October 2008. published online 08 December 2008.

Abstract

An intraluminal thrombus (ILT) forms in the majority of abdominal aortic aneurysms (AAAs). While the ILT has traditionally been perceived as a byproduct of aneurysmal disease, the mechanical environment within the ILT may contribute to the degeneration of the aortic wall by affecting biological events of cells embedded within the ILT. In this study, the drained secant modulus (E₅∼modulus at 5% strain) of ILT specimens (luminal, medial, and abluminal) procured from elective open repair was measured and compared using unconfined compression. Five groups of fibrin-based thrombus mimics were also synthesized by mixing various combinations of fibrinogen, thrombin, and calcium. Drained secant moduli were compared to determine the effect of the components’ concentrations on mimic stiffness. The stiffness of mimics was also compared to the native ILT. Preliminary data on the water content of the ILT layers and mimics was measured. It was found that the abluminal layer (E₅=19.3kPa) is stiffer than the medial (2.49kPa) and luminal (1.54kPa) layers, both of which are statistically similar. E₅ of the mimics (0.63, 0.22, 0.23, 0.87, and 2.54kPa) is dependent on the concentration of all three components: E₅ decreases with a decrease in fibrinogen (60–20 and 20–15mg/ml) and a decrease in thrombin (3–0.3 units/ml), and E₅ increases with a decrease in calcium (0.1–0.01M). E₅ from two of the mimics were not statistically different than the medial and luminal layers of ILT. A thrombus mimic with similar biochemical components, structure, and mechanical properties as native ILT would provide an appropriate test medium for AAA mechanobiology studies.