Journal of Biomechanics
Volume 42, Issue 3 , Pages 297-302 , 9 February 2009

Strain field in actin filament network in lamellipodia of migrating cells: Implication for network reorganization

  • Taiji Adachi

      Affiliations

    • Department of Mechanical Engineering and Science, Kyoto University, Yoshida-honmachi, Sakyo, Kyoto 606-8501, Japan
    • Computational Cell Biomechanics Team, VCAD System Research Program, RIKEN, Japan
    • Corresponding Author InformationCorresponding author at: Department of Mechanical Engineering and Science, Kyoto University, Yoshida-honmachi, Sakyo, Kyoto 606-8501, Japan. Tel./fax: +81757535216.
    • ,
  • Kennedy Omondi Okeyo

      Affiliations

    • Department of Mechanical Engineering and Science, Kyoto University, Yoshida-honmachi, Sakyo, Kyoto 606-8501, Japan
    • ,
  • Yoshimichi Shitagawa

      Affiliations

    • Department of Mechanical Engineering and Science, Kyoto University, Yoshida-honmachi, Sakyo, Kyoto 606-8501, Japan
    • ,
  • Masaki Hojo

      Affiliations

    • Department of Mechanical Engineering and Science, Kyoto University, Yoshida-honmachi, Sakyo, Kyoto 606-8501, Japan

,Accepted 1 November 2008.

References 

  1. Borisy GG, Svitkina TM. Actin machinery: pushing the envelope. Current Opinion in Cell Biology. 2000;12(1):104–112
  2. Costa KD, Hucker WJ, Yin FCP. Buckling of actin stress fibers: a new wrinkle in the cytoskeletal tapestry. Cell Motility and the Cytoskeleton. 2002;52(4):266–274
  3. Danuser G, Waterman-Storer CM. Quantitative fluorescent speckle microscopy of cytoskeleton dynamics. Annual Review of Biophysics and Biomolecular Structure. 2006;35:361–387
  4. De La Cruz EM, Pollard TD. Transient kinetic-analysis of rhodamine phalloidin binding to actin-filaments. Biochemistry. 1994;33(48):14387–14392
  5. Hayakawa K, Tatsumi H, Sokabe M. ADF/cofilin is involved in stress fiber disassembly by periodic stretching. Biophysics. 2003;43(S1):S162
  6. Higashida C, Miyoshi T, Fujita A, OcegueraYanez F, Monypenny J, Andou Y, et al. Actin polymerization-driven molecular movement of mDia1 in living cells. Science. 2004;303(5666):2007–2010
  7. Hu K, Ji L, Applegate KT, Danuser G, Waterman-Storer CM. Differential transmission of actin motion within focal adhesions. Science. 2007;315(5808):111–115
  8. Huang ZJ, Haugland RP, You WM, Haugland RP. Phallotoxin and actin binding assay by fluorescence enhancement. Analytical Biochemistry. 1992;200(1):199–204
  9. Jurado C, Haserick JR, Lee J. Slipping or gripping? Fluorescent speckle microscopy in fish keratocytes reveals two different mechanisms for generating a retrograde flow of actin. Molecular Biology of the Cell. 2005;16(2):507–518
  10. Kolega J. Effects of mechanical tension on protrusive activity and microfilament and intermediate filament organization in an epidermal epithelium moving in culture. Journal of Cell Biology. 1986;102(4):1400–1411
  11. Kovar DR, Harris ES, Mahaffy R, Higgs HN, Pollard TD. Control of the assembly of ATP- and ADP-actin by formins and profilin. Cell. 2006;124(2):423–435
  12. Lauffenburger DA, Horwitz AF. Cell migration: a physically integrated molecular process. Cell. 1996;84(3):359–369
  13. Lee J, Ishihara A, Theriot JA, Jacobson K. Principles of locomotion for simple-shaped cells. Nature. 1993;362(6416):167–171
  14. Lee J, Leonard M, Oliver T, Ishihara A, Jacobson K. Traction forces generated by locomoting keratocytes. Journal of Cell Biology. 1994;127(6):1957–1964
  15. Lombardi ML, Knecht DA, Dembo M, Lee J. Traction force microscopy in dictyostelium reveals distinct roles for myosin II motor and actin-crosslinking activity in polarized cell movement. Journal of Cell Science. 2007;120(9):1624–1634
  16. Mahaffy RE, Pollard TD. Influence of phalloidin on the formation of actin filament branches by Arp2/3 complex. Biochemistry. 2008;47(24):6460–6467
  17. McCullough BR, Blanchoin L, Martiel JL, De la Cruz EM. Cofilin increases the bending flexibility of actin filaments: implications for severing and cell mechanics. Journal of Molecular Biology. 2008;381(3):550–558
  18. Mitchison TJ, Cramer LP. Actin-based cell motility and cell locomotion. Cell. 1996;84(3):371–379
  19. Miyoshi T, Tsuji T, Higashida C, Hertzog M, Fujita A, Narumiya S, et al. Actin turnover-dependent fast dissociation of capping protein in the dendritic nucleation actin network: evidence of frequent filament severing. Journal of Cell Biology. 2006;175(6):947–955
  20. Okeyo KO, Shitagawa Y, Adachi T, Hojo M. Quantitative evaluation of strain field in the lamella region of cellular fragments from fish keratocytes. Journal of Biomechanics. 2006;39(S1):S244
  21. Palecek SP, Loftus JC, Ginsberg MH, Lauffenburger DA, Horwitz AF. Integrin–ligand binding properties govern cell migration speed through cell–substratum adhesiveness. Nature. 1997;385(6616):537–540
  22. Pollard TD. The cytoskeleton, cellular motility and the reductionist agenda. Nature. 2003;422(6933):741–745
  23. Pollard TD, Borisy GG. Cellular motility driven by assembly and disassembly of actin filaments. Cell. 2003;112(4):453–465
  24. Ponti A, Machacek M, Gupton SL, Waterman-Storer CM, Danuser G. Two distinct actin networks drive the protrusion of migrating cells. Science. 2004;305(5691):1782–1786
  25. Prass M, Jacobson K, Mogilner A, Radmacher M. Direct measurement of the lamellipodial protrusive force in a migrating cell. Journal of Cell Biology. 2006;174(6):767–772
  26. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, et al. Cell migration: integrating signals from front to back. Science. 2003;302(5651):1704–1709
  27. Sato K, Adachi T, Matsuo M, Tomita Y. Quantitative evaluation of threshold fiber strain that induces reorganization of cytoskeletal actin fiber structure in osteoblastic cells. Journal of Biomechanics. 2005;38(9):1895–1901
  28. Sato K, Adachi T, Shirai Y, Saito N, Tomita Y. Local disassembly of actin stress fibers induced by selected release of intracellular tension in osteoblastic cell. Journal of Biomechanical Science and Engineering. 2006;1(1):204–214
  29. Schaub S, Bohnet S, Laurent VM, Meister JJ, Verkhovsky AB. Comparative maps of motion and assembly of filamentous actin and myosin II in migrating cells. Molecular Biology of the Cell. 2007;18(10):3723–3732
  30. Svitkina TM, Borisy GG. Arp2/3 complex and actin depolymerizing factor cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia. Journal of Cell Biology. 1999;145(5):1009–1026
  31. Svitkina TM, Verkhovsky AB, McQuade KM, Borisy GG. Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation. Journal of Cell Biology. 1997;139(2):397–415
  32. Vallotton P, Gupton SL, Waterman-Storer CM, Danuser G. Simultaneous mapping of filamentous actin flow and turnover in migrating cells by quantitative fluorescent speckle microscopy. Proceedings of the National Academy of Sciences of the United States of America. 2004;101(26):9660–9665
  33. Vallotton P, Danuser G, Bohnet S, Meister JJ, Verkhovsky AB. Tracking retrograde flow in keratocytes: news from the front. Molecular Biology of the Cell. 2005;16(3):1223–1231
  34. Verkhovsky AB, Svitkina TM, Borisy GG. Network contraction model for cell translocation and retrograde flow. Cell Behaviour: Control and Mechanism of Motility. 1999;(65):207–222
  35. Watanabe N, Mitchison TJ. Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science. 2002;295(5557):1083–1086
  36. Waterman-Storer CM, Desai A, Bulinski JC, Salmon ED. Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells. Current Biology. 1998;8(22):1227–1230
  37. Willert CE, Gharib M. Digital particle image velocimetry. Experiments in Fluids. 1991;10:181–193

PII: S0021-9290(08)00563-0

doi: 10.1016/j.jbiomech.2008.11.012

Journal of Biomechanics
Volume 42, Issue 3 , Pages 297-302 , 9 February 2009

Article Tools

* Image Usage & Resolution