Journal of Biomechanics
Volume 43, Issue 1 , Pages 9-14 , 5 January 2010

Mechanics of the F-actin cytoskeleton

  • Jonathan Stricker

      Affiliations

    • Department of Physics, University of Chicago, USA
    • James Franck Institute, University of Chicago, USA
    • ,
  • Tobias Falzone

      Affiliations

    • Graduate Program in Biophysical Sciences, University of Chicago, USA
    • Institute for Biophysical Dynamics, University of Chicago, USA
    • ,
  • Margaret L. Gardel

      Affiliations

    • Department of Physics, University of Chicago, USA
    • James Franck Institute, University of Chicago, USA
    • Institute for Biophysical Dynamics, University of Chicago, USA
    • Corresponding Author InformationCorresponding author at: Gardel, M. James Franck Institute, Department of Physics, Institute for Biophysical Dynamics, University of Chicago, USA.

,Accepted 21 August 2009.

References 

  1. Achim B, Ulrich SS. Coupling biochemistry and mechanics in cell adhesion: a model for inhomogeneous stress fiber contraction. N. J. Phys. 2007;11:425
  2. Akin O, Mullins RD. Capping protein increases the rate of actin-based motility by promoting filament nucleation by the Arp2/3 complex. Cell. 2008;133(5):841–851
  3. Bendix PM, et al. A quantitative analysis of contractility in active cytoskeletal protein networks. Biophys. J. 2008;94(8):3126–3136
  4. Bischofs IB, et al. Filamentous network mechanics and active contractility determine cell and tissue shape. Biophys. J. 2008;95(7):3488–3496
  5. Bohnet S, et al. Weak force stalls protrusion at the leading edge of the lamellipodium. Biophys. J. 2006;90(5):1810–1820
  6. Broedersz CP, Storm C, MacKintosh FC. Nonlinear elasticity of composite networks of stiff biopolymers with flexible linkers. Phys. Rev. Lett. 2008;101(11):
  7. Bursac P, et al. Cytoskeletal remodelling and slow dynamics in the living cell. Nat. Mater. 2005;4(7):557–561
  8. Cameron LA, et al. Secrets of actin-based motility revealed by a bacterial pathogen. Nat. Rev. Mol. Cell Biol. 2000;1(2):110–119
  9. Carlier MF, et al. Actin-based motility as a self-organized system: mechanism and reconstitution in vitro. C. R. Biol. 2003;326(2):161–170
  10. Carlsson AE. Contractile stress generation by actomyosin gels. Phys. Rev. E. Stat. Nonlinear Soft Matter Phys. 2006;74(5 Pt 1):051912
  11. Chan CE, Odde DJ. Traction dynamics of filopodia on compliant substrates. Science. 2008;322(5908):1687–1691
  12. Charras GT, et al. Reassembly of contractile actin cortex in cell blebs. J. Cell. Biol. 2006;175(3):477–490
  13. Charras GT, et al. Life and times of a cellular bleb. Biophys. J. 2008;94(5):1836–1853
  14. Chaudhuri O, Parekh SH, Fletcher DA. Reversible stress softening of actin networks. Nature. 2007;445(7125):295–298
  15. Clarke M, Spudich JA. Nonmuscle contractile proteins: the role of actin and myosin in cell motility and shape determination. Annu. Rev. Biochem. 1977;46:797–822
  16. Cojoc D, et al. Properties of the force exerted by filopodia and lamellipodia and the involvement of cytoskeletal components. PLoS ONE. 2007;2(10):e1072
  17. Cramer LP. Organization and polarity of actin filament networks in cells: implications for the mechanism of myosin-based cell motility. Biochem. Soc. Symp. 1999;65:173–205
  18. Cramer LP, Mitchison TJ, Theriot JA. Actin-dependent motile forces and cell motility. Curr. Opinion Cell. Biol. 1994;6(1):82–86
  19. Deguchi S, Ohashi T, Sato M. Tensile properties of single stress fibers isolated from cultured vascular smooth muscle cells. J. Biomech. 2006;39(14):2603–2610
  20. Esue O, Tseng Y, Wirtz D. Alpha-actinin and filamin cooperatively enhance the stiffness of actin filament networks. PLoS ONE. 2009;4(2):e4411
  21. Fischer RS, et al. Local cortical tension by myosin II guides 3D endothelial cell branching. Curr. Biol. 2009;19(3):260–265
  22. Fletcher DA, Geissler PL. Active biological materials. Annu. Rev. Phys. Chem. 2009;60:469–486
  23. Footer MJ, et al. Direct measurement of force generation by actin filament polymerization using an optical trap. Proc. Natl. Acad. Sci. USA. 2007;104(7):2181–2186
  24. Gardel ML, et al. Elastic behavior of cross-linked and bundled actin networks. Science. 2004;304(5675):1301–1305
  25. Gardel ML, et al. Stress-dependent elasticity of composite actin networks as a model for cell behavior. Phys. Rev. Lett. 2006;96(8):088102
  26. Gardel ML, et al. Prestressed F-actin networks cross-linked by hinged filamins replicate mechanical properties of cells. Proc. Natl. Acad. Sci. USA. 2006;103(6):1762–1767
  27. Gardel ML, et al. Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed. J. Cell. Biol. 2008;183(6):999–1005
  28. Gardel ML, et al. Chapter 19: mechanical response of cytoskeletal networks. Methods Cell. Biol. 2008;89:487–519
  29. Giannone G, et al. Lamellipodial actin mechanically links myosin activity with adhesion-site formation. Cell. 2007;128(3):561–575
  30. Head DA, Levine AJ, MacKintosh FC. Distinct regimes of elastic response and deformation modes of cross-linked cytoskeletal and semiflexible polymer networks. Phys. Rev. E. Stat. Nonlinear Soft Matter Phys. 2003;68(6 Pt 1):061907
  31. Head DA, Levine AJ, MacKintosh FC. Deformation of cross-linked semiflexible polymer networks. Phys. Rev. Lett. 2003;91(10):108102
  32. Hotulainen P, Lappalainen P. Stress fibers are generated by two distinct actin assembly mechanisms in motile cells. J. Cell Biol. 2006;173(3):383–394
  33. Janmey PA, et al. Resemblance of actin-binding protein/actin gels to covalently crosslinked networks. Nature. 1990;345(6270):89–92
  34. Janson LW, Kolega J, Taylor DL. Modulation of contraction by gelation/solation in a reconstituted motile model. J. Cell Biol. 1991;114(5):1005–1015
  35. Janson LW, Sellers JR, Taylor DL. Actin-binding proteins regulate the work performed by myosin II motors on single actin filaments. Cell Motil. Cytoskeleton. 1992;22(4):274–280
  36. Jurado C, Haserick JR, Lee J. Slipping or gripping? Fluorescent speckle microscopy in fish keratocytes reveals two different mechanisms for generating a retrograde flow of actin. Mol. Biol. Cell. 2005;16(2):507–518
  37. Kasza KE, et al. The cell as a material. Curr. Opinion Cell Biol. 2007;19(1):101–107
  38. Kasza KE, et al. Nonlinear elasticity of stiff biopolymers connected by flexible linkers. Phys. Rev.E (Stat., Nonlinear, Soft Matter Phys.). 2009;79(4):041928–041935
  39. Kruse K, et al. Contractility and retrograde flow in lamellipodium motion. Phys. Biol. 2006;3(2):130–137
  40. Kumar S, et al. Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics. Biophys. J. 2006;90(10):3762–3773
  41. Le Clainche C, Carlier M-F. Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol. Rev. 2008;88(2):489–513
  42. Lieleg O, et al. Transient binding and dissipation in cross-linked actin networks. Phys. Rev. Lett. 2008;101(10):108101
  43. Lieleg O, Bausch AR. Cross-linker unbinding and self-similarity in bundled cytoskeletal networks. Phys. Rev. Lett. 2007;99(15):158105
  44. Lin CH, Forscher P. Growth cone advance is inversely proportional to retrograde F-actin flow. Neuron. 1995;14(4):763–771
  45. Lu L, et al. Mechanical properties of actin stress fibers in living cells. Biophys. J. 2008;95(12):6060–6071
  46. MacKintosh FC, Käs J, Janmey PA. Elasticity of semiflexible biopolymer networks. Phys. Rev. Lett. 1995;75(24):4425
  47. MacKintosh FC, Kas J, Janmey PA. Elasticity of semiflexible biopolymer networks. Phys. Rev. Lett. 1995;75(24):4425–4428
  48. Marston S, Weber A. Dissociation constant of the actin-heavy meromyosin subfragment-1 complex. Biochemistry. 2002;14(17):3868–3873
  49. Mitchison T, Kirschner M. Cytoskeletal dynamics and nerve growth. Neuron. 1988;1(9):761–772
  50. Naumanen P, Lappalainen P, Hotulainen P. Mechanisms of actin stress fibre assembly. J. Microsc. 2008;231(3):446–454
  51. Paul R, et al. Propagation of mechanical stress through the actin cytoskeleton towards focal adhesions: model and experiment. Biophys. J. 2008;94(4):1470–1482
  52. Peterson LJ, et al. Simultaneous stretching and contraction of stress fibers in vivo. Mol. Biol. Cell. 2004;15(7):3497–3508
  53. Pollard TD. Cytoskeletal functions of cytoplasmic contractile proteins. J. Supramol. Struct. 1976;5(3):317–334
  54. Prass M, et al. Direct measurement of the lamellipodial protrusive force in a migrating cell. J. Cell Biol. 2006;174(6):767–772
  55. Rosenbluth MJ, et al. Slow stress propagation in adherent cells. Biophys. J. 2008;95(12):6052–6059
  56. Senju Y, Miyata H. The role of actomyosin contractility in the formation and dynamics of actin bundles during fibroblast spreading. J. Biochem. 2009;145(2):137–150
  57. Solon J, et al. Fibroblast adaptation and stiffness matching to soft elastic substrates. Biophys. J. 2007;93(12):4453–4461
  58. Stachowiak M, O’Shaughnessy B. Kinetics of stress fibers. N. J. Phys. 2008;2008(2):025002
  59. Storm C, et al. Nonlinear elasticity in biological gels. Nature. 2005;435(7039):191–194
  60. Stossel TP. Contractile proteins in cell structure and function. Annu. Rev. Med. 1978;29:427–457
  61. Tharmann R, Claessens MMAE, Bausch AR. Viscoelasticity of isotropically cross-linked actin networks. Phys. Rev. Lett. 2007;98(8):088103
  62. Tharmann R, Claessens MM, Bausch AR. Viscoelasticity of isotropically cross-linked actin networks. Phys. Rev. Lett. 2007;98(8):088103
  63. Vale RD, Kreis T. Guidebook to the Cytoskeletal and Motor Proteins. second ed.. Oxford: Oxford University Press; 1999;
  64. Verkhovsky AB, Borisy GG. Non-sarcomeric mode of myosin II organization in the fibroblast lamellum. J. Cell Biol. 1993;123(3):637–652
  65. Verkhovsky AB, Svitkina TM, Borisy GG. Myosin II filament assemblies in the active lamella of fibroblasts: their morphogenesis and role in the formation of actin filament bundles. J. Cell Biol. 1995;131(4):989–1002
  66. Wachsstock DH, Schwartz WH, Pollard TD. Affinity of alpha-actinin for actin determines the structure and mechanical properties of actin filament gels. Biophys. J. 1993;65(1):205–214
  67. Wachsstock DH, Schwarz WH, Pollard TD. Cross-linker dynamics determine the mechanical properties of actin gels. Biophys. J. 1994;66(3 Pt 1):801–809
  68. Wang N, et al. Cell prestress. I. Stiffness and prestress are closely associated in adherent contractile cells.. Am. J. Physiol. Cell Physiol. 2002;282(3):C606–616
  69. Wang YL. Flux at focal adhesions: slippage clutch, mechanical gauge, or signal depot. Sci. STKE. 2007;2007(377):pe10
  70. Ward SM, et al. Dynamic viscoelasticity of actin cross-linked with wild-type and disease-causing mutant alpha-actinin-4. Biophys. J. 2008;95(10):4915–4923
  71. Wilhelm J, Frey E. Elasticity of stiff polymer networks. Phys. Rev. Lett. 2003;91(10):108103
  72. Xu J, Tseng Y, Wirtz D. Strain hardening of actin filament networks. Regulation by the dynamic cross-linking protein alpha-actinin. J. Biol. Chem. 2000;275(46):35886–35892

PII: S0021-9290(09)00494-1

doi: 10.1016/j.jbiomech.2009.09.003

Journal of Biomechanics
Volume 43, Issue 1 , Pages 9-14 , 5 January 2010

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