Journal of Biomechanics
Volume 43, Issue 12 , Pages 2315-2320 , 26 August 2010

Quantitative, structural, and image-based mechanical analysis of nonunion fracture repaired by genetically engineered mesenchymal stem cells

  • Ilan Kallai

      Affiliations

    • Skeletal Biotech Laboratory, The Hebrew University – Hadassah Faculty of Dental Medicine, PO Box 12272, Ein Kerem, Jerusalem 91120, Israel
    • Both authors equally contributed to this manuscript.
    • ,
  • G. Harry van Lenthe

      Affiliations

    • Institute for Biomechanics, ETH Zurich, Zurich, Switzerland
    • Division of Biomechanics, K.U. Leuven, Leuven, Belgium
    • Both authors equally contributed to this manuscript.
    • ,
  • Davide Ruffoni

      Affiliations

    • Institute for Biomechanics, ETH Zurich, Zurich, Switzerland
    • ,
  • Yoram Zilberman

      Affiliations

    • Skeletal Biotech Laboratory, The Hebrew University – Hadassah Faculty of Dental Medicine, PO Box 12272, Ein Kerem, Jerusalem 91120, Israel
    • ,
  • Ralph Müller

      Affiliations

    • Institute for Biomechanics, ETH Zurich, Zurich, Switzerland
    • ,
  • Gadi Pelled

      Affiliations

    • Skeletal Biotech Laboratory, The Hebrew University – Hadassah Faculty of Dental Medicine, PO Box 12272, Ein Kerem, Jerusalem 91120, Israel
    • Department of Surgery and Cedars Sinai Regenerative Medicine Institute (CS-RMI), Cedars Sinai Medical Center, Los Angeles, CA, USA
    • ,
  • Dan Gazit

      Affiliations

    • Skeletal Biotech Laboratory, The Hebrew University – Hadassah Faculty of Dental Medicine, PO Box 12272, Ein Kerem, Jerusalem 91120, Israel
    • Department of Surgery and Cedars Sinai Regenerative Medicine Institute (CS-RMI), Cedars Sinai Medical Center, Los Angeles, CA, USA
    • Corresponding Author InformationCorresponding author at: Skeletal Biotech Laboratory, The Hebrew University – Hadassah Faculty of Dental Medicine, PO Box 12272, Ein Kerem, Jerusalem 91120, Israel. Tel.: +97226757627; fax: +97226757628.

,Accepted 24 April 2010.

References 

  1. Arbenz P, van Lenthe GH, Mennel U, Müller R, Sala M. A scalable multi-level preconditioner for matrix-free μ-finite element analysis of human bone structures. Int. J. Numer. Methods Eng. 2008;73:927–947
  2. Gazit D, Turgeman G, Kelley P, Wang E, Jalenak M, Zilberman Y, et al. Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone: a novel cell-mediated gene therapy. J. Gene Med. 1999;1:121–133
  3. Hasharoni A, Zilberman Y, Turgeman G, Helm GA, Liebergall M, Gazit D. Murine spinal fusion induced by engineered mesenchymal stem cells that conditionally express bone morphogenetic protein-2. J. Neurosurg. Spine. 2005;3:47–52
  4. Hildebrand T, Laib A, Müller R, Dequeker J, Ruegsegger P. Direct three-dimensional morphometric analysis of human cancellous bone: microstructural data from spine, femur, iliac crest, and calcaneus. J. Bone Miner. Res. 1999;14:1167–1174
  5. Homminga J, McCreadie BR, Weinans H, Huiskes R. The dependence of the elastic properties of osteoporotic cancellous bone on volume fraction and fabric. J. Biomech. 2003;36:1461–1467
  6. Hsu WK, Sugiyama O, Park SH, Conduah A, Feeley BT, Liu NQ, et al. Lentiviral-mediated bmp-2 gene transfer enhances healing of segmental femoral defects in rats. Bone. 2007;40:931–938
  7. Kabel J, van Rietbergen B, Dalstra M, Odgaard A, Huiskes R. The role of an effective isotropic tissue modulus in the elastic properties of cancellous bone. J. Biomech. 1999;32:673–680
  8. Ladd AJ, Kinney JH, Haupt DL, Goldstein SA. Finite-element modeling of trabecular bone: comparison with mechanical testing and determination of tissue modulus. J. Orthop. Res. 1998;16:622–628
  9. Lieberman JR, Le LQ, Wu L, Finerman GA, Berk A, Witte ON, et al. Regional gene therapy with a bmp-2-producing murine stromal cell line induces heterotopic and orthotopic bone formation in rodents. J. Orthop. Res. 1998;16:330–339
  10. Miller LM, Little W, Schirmer A, Sheik F, Busa B, Judex S. Accretion of bone quantity and quality in the developing mouse skeleton. J. Bone Miner. Res. 2007;22:1037–1045
  11. Morgan EF, Mason ZD, Chien KB, Pfeiffer AJ, Barnes GL, Einhorn TA, et al. Micro-computed tomography assessment of fracture healing: relationships among callus structure, composition, and mechanical function. Bone. 2009;44:335–344
  12. Moutsatsos IK, Turgeman G, Zhou S, Kurkalli BG, Pelled G, Tzur L, et al. Exogenously regulated stem cell-mediated gene therapy for bone regeneration. Mol. Ther. 2001;3:449–461
  13. Müller R, Ruegsegger P. Micro-tomographic imaging for the nondestructive evaluation of trabecular bone architecture. Stud. Health Technol. Inf. 1997;40:61–79
  14. Müller R, Van Campenhout H, Van Damme B, Van Der Perre G, Dequeker J, Hildebrand T, et al. Morphometric analysis of human bone biopsies: a quantitative structural comparison of histological sections and micro-computed tomography. Bone. 1998;23:59–66
  15. Shefelbine SJ, Simon U, Claes L, Gold A, Gabet Y, Bab I, et al. Prediction of fracture callus mechanical properties using micro-CT images and voxel-based finite element analysis. Bone. 2005;
  16. Sheyn D, Pelled G, Zilberman Y, Talasazan F, Frank JM, Gazit D, et al. Nonvirally engineered porcine adipose tissue-derived stem cells: use in posterior spinal fusion. Stem Cells. 2008;26:1056–1064
  17. Tai K, Pelled G, Sheyn D, Bershteyn A, Han L, Kallai I, et al. Nanobiomechanics of repair bone regenerated by genetically modified mesenchymal stem cells. Tissue Eng. Part A. 2008;14:1709–1720
  18. Turgeman G, Pittman DD, Muller R, Kurkalli BG, Zhou S, Pelled G, et al. Engineered human mesenchymal stem cells: a novel platform for skeletal cell mediated gene therapy. J. Gene Med. 2001;3:240–251
  19. van Lenthe GH, Voide R, Boyd SK, Muller R. Tissue modulus calculated from beam theory is biased by bone size and geometry: implications for the use of three-point bending tests to determine bone tissue modulus. Bone. 2008;43:717–723
  20. van Rietbergen B, Weinans H, Huiskes R, Odgaard A. A new method to determine trabecular bone elastic properties and loading using micromechanical finite-element models. J. Biomech. 1995;28:69–81
  21. Zilberman Y, Kallai I, Gafni Y, Pelled G, Kossodo S, Yared W, et al. Fluorescence molecular tomography enables in vivo visualization and quantification of nonunion fracture repair induced by genetically engineered mesenchymal stem cells. J. Orthop. Res. 2008;26:522–530

PII: S0021-9290(10)00243-5

doi: 10.1016/j.jbiomech.2010.04.031

Journal of Biomechanics
Volume 43, Issue 12 , Pages 2315-2320 , 26 August 2010

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